MiDAS Global Database Workshop
20.06.2019 kl. 08.30 - 21.06.2019 kl. 15.00
MiDAS (Microbial Database for Activated Sludge) is a central collaborative workspace available for researchers and wastewater treatment practitioners to facilitate a better understanding of the microbial ecology of the engineered ecosystems of activated sludge plants, anaerobic digesters, and related wastewater treatment systems.
The ambition of the field guide (http://midasfieldguide.org/) is to summarize all knowledge about the physiology and ecology of the important microorganisms present in these systems. Integral to this goal is the ability to link an organism’s name with its putative function. To achieve this, a complete list of all microbes in these systems is necessary. Although originally based on a decade long study of Danish wastewater treatment plants and anaerobic digesters, MiDAS was expanded in 2018 to include microbes present in wastewater treatment systems and anaerobic digesters from all over the world. The aim of the global database project is to provide a (near) complete list of all microbes in the systems, and assign unique sequence IDs to these to facilitate a global common language for all future studies and to connect their identity and function in the MiDAS Field Guide.
During the workshop, the results from the MiDAS Global Database of wastewater treatment systems will be presented and discussed with special focus on the catalogue of microorganisms and new, expanded MiDAS taxonomy, interesting cases and practical application. Furthermore, Professor Tom P. Curtis (Newcastle University, United Kingdoms) and Dr. Rohan Williams (SCELSE, Singapore) will present time series data analyses.
The workshop is held at:
Department of Chemistry and Bioscience
Fredrik Bajers Vej 7H, Auditorium 1.002 (ground level)
9220 Aalborg East
Thursday, June 20
9:00 Welcome and introduction, Per H. Nielsen
- Overall aim and approach
- Advantages in using ecosystems-specific reference database
9:30 The new MiDAS reference database, Morten S. Dueholm
- Long 16S rRNA gene sequences – provide ID at species level
- New MiDAS taxonomy
10:00 Amplicon sequencing: how to handle amplicon data with the new reference database, Kasper S. Andersen
- OTUs or ASVs? A question of resolution
- Application of the new MiDAS taxonomy
11:00 Overview of the Global Database of Wastewater Treatment Systems, Per H. Nielsen
11:30 Case 1: Filamentous bacteria across the world, Marta Nierychlo
13:00 Case 2: PAOs/GAOs across the world, Per H. Nielsen
13:30 Case 3: Nitrifiers across the world, Ditte M. Hansen
14:00 Importance of protocols (sampling, extraction, primers, etc.), Susan H, Hansen /Søren M. Karst
14:30 Global Database of anaerobic digestion – status, Vibeke R. Jørgensen
15:30 Discussion + Q/A, Per. H. Nielsen
18:30 Dinner in Aalborg city center
Friday, June 21
8:30 MiDAS Field Guide – web-page information, Marta Nierychlo
9:00 Linking ID to function by meta-omics, Mads Albertsen/Caitlin Singleton
10:30 Time series analyses, Tom Curtis, Newcastle University
11:15 Analysis and interpretation of time series data from full-scale WWTPs, Rohan Williams, SCELSE
13:00 Using the MiDAS information – practical experience with 25 Danish plants, Marta Nierychlo
13:30 MiDAS future – discussion, Per. H. Nielsen
Please note that the program is subject to change, and may be updated up to the workshop.
For your information
Please note that the workshop will be documented by video/photos. This material may be used on print and in digital media formats (e.g., presentations for webinars, and photos for printed matter, homepage and social media). If you do not wish to appear on these formats, please let us know.
1530 DKK (approximately €200), which covers lunches, coffee/tea and water during the workshop, as well as dinner Thursday evening. If you have any special dietary needs or restrictions, please let us know.
25% Danish VAT is included in the price.
Participation in the workshop is free for collaborators who have provided samples for the MiDAS global database (please note that this registration is subject to verification by the organizers).
Vibeke R. Jørgensen
+45 9940 7243
Per H. Nielsen
Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark,
31.05.2019 kl. 23.00